Biotin Protein (HRP)

N° du produit ABIN964124

N° du produit (Fournisseur): b000-03

Cette protéine Biotin est produite dans Synthetic.

Aperçu rapide pour Biotin Protein (HRP) (ABIN964124)

Antigène: Biotin

Origine: Chemical

Source: Synthetic

Application: Dot Blot (DB), ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Détail du produit

Purification/Conjugué: Cette Biotin protéine est marqué à la HRP.

N° du produit (Fournisseur): b000-03

Fournisseur: Rockland

Fonction: Biotin Peroxidase Conjugated

Purification: Biotin Peroxidase Conjugated was prepared from chromatographically purified biotin. Biotin Peroxidase Conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Biotin.

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Biotin Peroxidase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.

Western Blot Dilution: 1:10,000 - 1:40,000

ELISA Dilution: 1:20,000 - 1:200,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent) (in 5.0 mL)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Biotin Peroxidase Conju Expiration date is one (1) year from date of receipt.

Date de péremption: 12 months

Détail du antigène

Antigène: Biotin

Classe de substances: Chemical

Sujet:

Synonyms: Vitamin H, Coenzyme R, Vitamin B7, Biotin Peroxidase Conjugated, Biotin HRP Conjugated

Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptav